351 Development and characterization of an immune 3D model mimicking psoriasis

Psoriasis is a common chronic skin disease characterized by pro-inflammatory environment mediated keratinocytes and both innate adaptative immune responses. This inflammation mostly driven the IL-17/IL-23 axis resulting in production of inflammatory factors (chemokines antimicrobial peptides) keratinocytes. These mediators lead to an amplification loop consequently epidermal hyperplasia. To our knowledge, very few 3D models involving lymphocyte T-helper cell type 17 (LTh17) reproduce accurately psoriasis features. The aim this work was develop immunocompetent model reproducing biological characteristics psoriatic lesional skin. As physiopathology interplay LTh17 keratinocytes, we developed that integrated these types. purpose, were differentiated from naive T cells rigorously characterized. Using ELISA assay flow cytometry, confirmed functional differentiation with relevant Th17 cytokines (IL-17A IL-17F). Then, co-cultured reconstructed epidermis. Our recapitulated features secretion characteristic (IL-17A, IL-17F), chemokines (CXCL1, CXCL8, CCL20) peptide (S100A7). At last, psoriasis-mimicking showed alteration keratinocyte leading morphological changes (acanthosis). model’s functionality assessed using anti-IL-17A neutralizing antibody. Thanks communication between compromised succeeded characteristics, including defects dysregulation response. could be suitable for screening natural therapeutic molecules dedicated treatment.